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KMID : 0923620120120050213
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Immune Network
2012 Volume.12 No. 5 p.213 ~ p.216
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Polyacetylene Compound from Cirsium japonicum var. ussuriense Inhibited Caspase-1-mediated IL-1β Expression
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Shim Hong
Moon Jung-Sun
Lee Sook-Yeon
Yim Dong-Sool
Kang Tae-Jin
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Abstract
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Our previous report showed that polyacetylene compound, 1-Heptadecene-11, 13-diyne-8, 9, 10-triol (PA) from the root of Cirsium japonicum var. ussuriense has anti-inflammatory activity. In this study we investigated the role of the PA as inhibitor of caspase-1, which converts prointerleukin-1β (proIL-1β) to active IL-1β and is activated by inflammasome involved in the inflammatory process. We tested the effect of PA on the production of pro-inflammatory cytokines, IL-1β in murine macrophage cell line, RAW264.7. PA inhibited lipopolysaccharide (LPS)-induced IL-1β production by macrophages at a dose dependent manner. PA also suppressed the activation of caspase-1. The mRNA level of ASC (apoptosis- associated spec-like protein containing a CARD), an important adaptor protein of inflammasome, was decreased in the PA treated group. Therefore our results suggest that the anti-inflammatory effect of PA is due to inhibit the caspase-1 activation.
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KEYWORD
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Polyacetylene compound, Anti-inflammatory activity, Caspase-1, Inflammasome, Cirsium japonicum
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